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The following agencies offer HIV testing. In most cases, HIV testing is free. However, you should check with the agency to be sure. You can also call the Delaware AIDS Hotline at 800-422-0429 to find a site closest to you.
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| State Agencies | Location | Telephone |
| Division of Public Health (Referral) | Statewide | 302-739-3032 |
| Laurel State Service Center | Laurel | 302-875-2289 |
| Middletown Health Unit | Middletown | 302-378-5200 |
| Milford State Service Center | Milford | 302-422-1390 |
| Northeast State Service Center | Wilmington | 302-577-3536 |
| Porter State Service Center | Wilmington | 302-577-3521 |
| Shipley State Service Center | Seaford | 302-628-2000 |
| Sussex County Health Unit | Georgetown | 302-856-5184 |
| VA Medical Center | Wilmington | 302-944-2511 |
| | | Or 800-461-8262 |
| Williams State Service Center | Dover | 302-739-4729 |
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| Private Agencies | Location | Telephone |
| AIDS Delaware | Wilmington | 302-652-6776 |
| Beautiful Gate Outreach Center | Wilmington | 302-472-3002 |
| Beebe Medical Center | Lewes | 302-645-3560 |
| Brandywine Counseling | Wilmington | 302-655-9880 ext. 24 |
| CAMP Safe | Rehoboth | 227-5620 |
| Christiana Care - HIV Community Program | Wilmington | 302-428-2538 |
| Christiana Care - HIV Community Program (Kent) | Smyrna | 302-739-5610 |
| Christiana Care - HIV Community Program (Sussex) | Georgetown | 302-856-5135 |
| Christiana Care - Women's Health (HIV Screening) | Newark | 302-733-6510 |
| Christiana Care - Women's Health (HIV Screening) | Wilmington | 302-428-4414 |
| Christiana Care - Women's Health (HIV Screening) | Middletown | 302-733-6510 |
| Claymont Community Center | Claymont | 302-652-6776 |
| DelMarVa Rural Ministries (MATCH Van Mobile Unit) | Dover | 302-678-2000 |
| Each One, Teach One; | Wilmington | 658-0217 |
| Henrietta Johnson Medical Center | Wilmington | 302-479-9693 |
| | | Or 655-6187 |
| Kent County Counseling | Dover | 302-735-7790 |
| La Red Health Center | Georgetown | 302-855-1233 |
| Latin American Community Center (Spanish-speaking) | Wilmington | 302-655-7338 |
| Planned Parenthood ($15 fee) | Claymont | 302-798-8000 |
| Planned Parenthood ($15 fee) | Dover | 302-678-5200 |
| Planned Parenthood ($15 fee) | Newark | 302-731-7801 |
| Planned Parenthood($15 fee) | Rehoboth | 645-2737 |
| Sussex County AIDS Committee | Rehoboth Beach | 302-644-1090 |
| Sussex County Counseling | Georgetown | 302-855-9158 |
| Threshold, Inc. | Georgetown | 302-856-1835 |